Linear Range Determination Overview

Each linear range workflow will help you identify the combined linear range for your experiment – the range of sample loading that produces a linear, proportional signal response for your target and your internal loading control. For example, a two-fold increase in sample loading is expected to produce an approximately two-fold increase in band intensity.

Before Quantitative Western blot analysis, you should determine the linear range of detection for your target and internal loading control. For accurate analysis, the amount of sample you load must produce a linear signal response for both the target and the loading control.

• Within the linear range of detection, signal intensity is proportional to the amount of sample loaded.
• Above and below the linear range, signal intensity does not accurately indicate the abundance of your target or internal loading control.

Serial dilutions of your sample are used to find the combined linear range.

See Finding the Combined Linear Range with Empiria Studio for more information about why the linear range needs to be determined and how linearity and proportionality are determined in Empiria Studio.